Sivas Bilim ve Teknoloji Üniversitesi, Tarım Bilimleri ve Teknoloji Fakültesi, Tarla Bitkileri Bölümü, Sivas
Abstract
This study aimed to evaluate the effects of different heavy metals (nickel [Ni], copper [Cu], cadmium [Cd], and lead [Pb]) on the early development of Petroselinum crispum (parsley), which is grown in open fields and has a high risk of exposure to heavy metal pollution under in vitro conditions. Heavy metal stress was induced in Murashige-Skoog (MS) nutrient medium at three different concentrations of 100, 300, and 500 μM for each metal. Multidimensional parameters such as germination rate, shoot and root length, fresh and dry biomass values, seedling growth indices (I and II), dry weight root:shoot ratio, tolerance indices, and relative germination were evaluated. Overall, the findings demonstrated a dose-dependent suppression of plant growth with increasing concentrations. Germination rates were 89.50 % and 90.00 % in the control and Ni-100 treatments, respectively, whereas they decreased to 28.67 % and 32.00 % in the Pb-500 and Cd-500 treatments, respectively. In addition, the root length tolerance index was determined as 13.62 % in Cd-500 and 16.62 % in Pb-500. Cd and Pb were the metals that most strongly suppressed germination and root development at high doses. Cu and Ni, however, showed relatively more tolerable effects at lower doses. In conclusion, this study, conducted under in vitro stress conditions, comparatively revealed the sensitivity levels of parsley plants to heavy metal toxicity.
KARA, E. (2026). Effects of Heavy Metal Stress on in vitro Germination and Seedling Growth of Parsley. ISPEC Journal of Agricultural Sciences, 10(2), 631–642. https://doi.org/10.5281/zenodo.20568509
📄Acila, S., Derouiche, S., Allioui, N., 2024. Embryo growth alteration and oxidative stress responses in germinating Cucurbita pepo seeds exposed to cadmium and copper toxicity. Scientific Reports, 14(1): 8608.
📄Ahmed, W.E., Almutairi, A.A., Almujaydil, M.S., Algonaiman, R., Mousa, H.M., Alhomaid, R.M., 2025. Nutraceutical potential of parsley (Petroselinum crispum Mill.): Comprehensive overview. Italian Journal of Food Science, 37(1):194-209.
📄Al, G.E., 2019. A study on the yield of parsley crops (Petroselinum crispum Mill.) grown in different environments during the harvest times and on the changes in the yield criteria. MSc thesis, Tokat Gaziosmanpaşa University, Institute of Natural and Applied Sciences, Tokat.
📄Alawadhi, N., Abass, K., Khaled, R., Osaili, T.M., Semerjian, L., 2024. Heavy metals in spices and herbs from worldwide markets: A systematic review and health risk assessment. Environmental Pollution, 362: 124999.
📄Aydemir, Ö.E., Kocaman, A., Korkmaz, K., Özkutlu, F., 2025. The effect of soil and foliar nitrogen applications on Cd toxicity of durum wheat (Triticum turgidum L.) grown in low and high cadmium contaminated soils. Journal of Crop Health, 77(1): 7.
📄Baktemur, G., 2022. In vitro koşullar altında farklı konsantrasyonlarda hazırlanan kurşun (Pb) ağır metalinin roka (Eruca sativa L.) bitkisinin gelişimi üzerine etkilerinin saptanması. ISPEC Journal of Agricultural Sciences, 6(4): 828-834.
📄Baran, O., Kara, E., Karaköy, T., Baktemur, G., 2022. Determination of the effects of some heavy metals applied in vitro conditions on the development of cucumber (Cucumis sativus L.). In 8th International Mardin Artuklu Scientific Researches Conference, Conference Proceedings Book, June 4-6, Mardin, s. 1296-1314.
📄Bekiş, P., Ergül, B., Kara, E., Baktemur, G., 2025. Evaluation of the effects of cobalt concentrations on the growth of bean (Phaseolus vulgaris) varieties under in vitro conditions. International Journal of Agriculture Environment and Food Sciences, 9(4): 999-1008.
📄Bibi, A., Farooq, U., Mirza, N., Khan, A., Sarwar, R., Alam, A., Mahmood, Q., 2014. Excessive chromium may cause dietary toxicity in parsley (Petroselinum crispum). Toxicological & Environmental Chemistry, 96(2): 287-295.
📄Dursun, E. 2023. Metotreksat ile oluşturulan beyin ve bağırsak hasarı üzerine maydanoz (Petroselinum crispum) ekstresinin etkilerinin incelenmesi. Yüksek Lisans Tezi, Marmara Üniversitesi, Sağlık Bilimleri Enstitüsü, İstanbul.
📄Dghaim, R., Al Khatib, S., Rasool, H., Ali Khan, M., 2015. Determination of heavy metals concentration in traditional herbs commonly consumed in the United Arab Emirates. Journal of environmental and public health, (1): 973878.
📄El-Sappah, A.H., Zhu, Y., Huang, Q., Chen, B., Soaud, S.A., Abd Elhamid, M.A., Yan, K., Li, J., El-Tarabily, K.A. 2024. Plants’ molecular behavior to heavy metals: from criticality to toxicity. Frontiers in Plant Science, 15: 1423625.
📄Ertaş, B., Turan, F.B., Özbeyli, D., Yanardağ, R., Saçan, Ö., Şener, G., 2021. Protective effects of Petroselinum crispum (Parsley) extract against methotrexate-induced hepatotoxicity. European Journal of Biology, 80(2): 173-178.
📄Farah, H., Elbadrawy, E., Al-Atoom, A.A., 2015. Evaluation of anti-oxidant and antimicrobial activities of ethanolic extracts of parsley (Petroselinum erispum) and coriander (Coriandrum sativum) plants grown in Saudi Arabia. International Journal of Advanced Research, 3(4): 1244-1255.
📄Gutarowska, B., Szulc, J., Jastrząbek, K., Kręgiel, D., Śmigielski, K., Cieciura-Włoch, W., Mroczyńska-Florczak, M., Liszkowska, W., Rygała, A., Berłowska, J., 2023. Effectiveness of ozonation for ımproving the microbiological safety of fresh-cut parsley (Petroselinum crispum) leaves. Applied Sciences, 13(15): 8946.
📄Gündeş, F. A., Sönmez, İ., 2021. Effect of phosphorus on the alleviation of boron toxicity in tomato (Lycopersicon esculentum L.) plant. Journal of Elementology, 26(4): 1053-1063.
📄Hussain, J., Saeed, W., Naqvi, T.A., Shah, M.M., Ahmad, R., Hassan, A., Mahmood, Q., 2015. Dietary toxicity of lead and hyper-accumulation in Petroselinum crispum. Arabian Journal for Science and Engineering, 40(7): 1819-1824.
📄Islam, E., Liu, D., Li, T., Yang, X., Jin, X., Mahmood, Q., Tian, S., Li, J., 2008. Effect of Pb toxicity on leaf growth, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. Journal of Hazardous Materials, 154(1-3): 914-926.
📄Hou, D., Jia, X., Wang, L., McGrath, S.P., Zhu, Y. G., Hu, Q., Zhao, F.J., Bank, M.S., O’Connor, D., Nriagu, J., 2025. Global soil pollution by toxic metals threatens agriculture and human health. Science, 388(6744): 316-321.
📄Kalsa, K.K., Abebie, B., 2012. Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp. dasycarpa (Ten.). African Journal of Agricultural Research, 7(21): 3202-3208.
📄Kara, E., Baktemur, G., 2022. Determining the effects of some heavy metal applications on white cabbage (Brassica oleracea var. capitata F. alba) in in vitro conditions. Ege 6th International Conference on Applied Sciences, Conference Proceedings Book, 10-11September, İzmir, s. 254-262.
📄Kara, E., 2025. Polietilen glikol (PEG) ile oluşturulan kuraklık stresine karşı farklı turp (Raphanus sativus L.) çeşitlerinin in vitro tepkileri. Ordu Üniversitesi Bilim ve Teknoloji Dergisi, 15(2): 372-386.
📄Kavvadias, V., Paschalidis, C., Vavoulidou, E., Petropoulos, D., Koriki, A., 2012. Effects of soil amended with cadmium and lead on growth, yield, and metal accumulation and distribution in parsley. Communications in Soil Science and Plant Analysis, 43(1-2): 161-175.
📄Kumar, B., Verma, S.K., Ram, G., Singh, H.P., 2012. Temperature relations for seed germination potential and seedling vigor in palmarosa (Cymbopogon martinii). Journal of Crop Improvement, 26(6): 791–801.
📄Kuru, İ.S., Orcan, P., 2025. Biochemical responses in Salvia limbata CA Mey. towards mercury and lead induced in vitro oxidative stress. Scientific Reports, 15(1): 32119.
📄Mancak, Z., Baktemur, G., 2023. Effects of nutrient media ıncluding heavy metals at different concentrations in in vitro conditions on the growth of squash (Cucurbita pepo L.). Türk Doğa ve Fen Dergisi, 12(1): 30-36.
📄Maodaa, S.N., Allam, A.A., Ajarem, J., Abdel-Maksoud, M.A., Al-Basher, G.I., Wang, Z.Y., 2016. Effect of parsley (Petroselinum crispum, Apiaceae) juice against cadmium neurotoxicity in albino mice (Mus musculus). Behavioral and Brain Functions, 12(1): 6.
📄Meharg, A.A., Meharg, C., 2021. The pedosphere as a sink, source, and record of anthropogenic and natural arsenic atmospheric deposition. Environmental Science & Technology, 55(12): 7757-7769.
📄Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
📄Nasircilar, A.G., Ulukapi, K., Topcuoglu, B., Kurubas, S., Erkan, M., 2024. Salt and heavy metal stress responses and metal uptake potentials of some leafy vegetables. Agrosystems, Geosciences & Environment, 7(1): e20487.
📄Nawaz, I., Mehboob, A., Khan, A.H.A., Naqvi, T.A., Bangash, N., Aziz, S., Khan, W., Shahzadi, İ., Barros, R., Ullah, K., Shah, M.M., 2024. Higher cadmium and zinc accumulation in parsley (Petroselinum crispum) roots activates its antioxidants defense system. Biometals, 37(1): 87-100.
📄Pereira, G.J.G., Molina, S.M.G., Lea, P., Azevedo, R.A.D., 2002. Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea. Plant and Soil, 239(1): 123-132.
📄Sakthivelu, G.M.K.A., Devi, M.A., Giridhar, P., Rajasekaran, T., Ravishankar, G.A., Nedev, T., Kosturkova, G., 2008. Drought-induced alterations in growth, osmotic potential and in vitro regeneration of soybean cultivars. General and Applied Plant Physiology, 34(1-2): 103-112.
📄Singh, P., Pandey, A., 2025. Indian knowledge system for sustainable mitigation of heavy metal effects in plants. IJSAT-International Journal on Science and Technology, 16(4).
📄Smýkal, P., Vernoud, V., Blair, M.W., Soukup, A., Thompson, R.D., 2014. The role of the testa during development and in establishment of dormancy of the legume seed. Frontiers in Plant Science, 5(1): 351.
📄Ulusu, Y., Öztürk, L. Elmastaş, M., 2017. Antioxidant capacity and cadmium accumulation in parsley seedlings exposed to cadmium stress. Russian Journal of Plant Physiology, 64: 883–888
📄Vega, A.S., Arce, G., Rivera, J.I., Acevedo, S.E., Reyes-Paecke, S., Bonilla, C.A., Pastén, P., 2022. A comparative study of soil metal concentrations in chilean urban parks using four pollution indexes. Applied Geochemistry, 141: 105230.
📄Wang, N., Chen, H., Tian, Y., 2024. Effects of nickel, lead, and copper stress on the growth and biochemical responses of Aegilops tauschii seedlings. Scientific Reports, 14: 24832.
📄Wu, Y., An, T., Gao, Y., Kuang, Q., Liu, S., Liang, L., Xu, B., Zhang, S., Deng, X., Chen, Y., 2023. Genotypic variation in the tolerance to moderate cadmium toxicity among 20 maize genotypes with contrasting root systems. Journal of the Science of Food and Agriculture, 103(5): 2618-2630.
📄Yılmaz, B., Çilingir Tütüncü, A., Saka, A.K., Demirkaya, S., Yılmaz, H., Ozer, H., 2024. Effects of different nutrient inputs in organic parsley cultivation. Biological Agriculture & Horticulture, 40(3): 161-172.
📄Zhao, X., Joo, J.C., Kim, D., Lee, J., Kim, J.Y., 2016. Estimation of the seedling vigor index of sunflowers treated with various heavy metals. Journal of Bioremediation & Biodegradation, 7(3): 1-6.
