In Vitro Propagation of Ornothogalum umbellatum
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https://doi.org/10.5281/zenodo.10225471Keywords:
Ornithogalum umbellatum, in vitro propagation, rootingAbstract
The objective of this study is to determine the propagation performance of Ornithogalum umbellatum, which is among the geophytes of our country, by tissue culture. The study was conducted in the tissue culture laboratory of the Field Crops Department of Agricultural Faculty of the Ege University. Bulbs explants of Ornithogalum umbellatum were used as genetic material in the study. Bulb explants were cultured in different cytokinins for the rejeneration in vitro conditions. The media of different levels of MS+Z (Zeatin) (0.5; 1.0 ve 2.0 mg L-1), MS +TDZ (Thidazuron) (3.0 ve 4.0 mg L-1) ve MS+BAP (6-Benzilaminopurin) (4.0 mg L-1) were used for in vitro propagation. There was no difference in media of Z, TDZ and BAP for the number of shoots. The medium of 0.5 m L-1 Z had the highest mean in terms of shoot length as 10.2 cm. For root number and root length were found to be successful in 0.5 mg L-1 and 1.0 mg L-1 Z but root formation was not achieved in the other media. Developing shoots were sub cultured in different amounts of IBA for root formation. While there was no difference in the IBA media for root number, 0.5 mg L-1 and 1.0 mg L-1 IBA had the highest mean in terms of root length. Bulbs explants of Ornithogalum umbellatum were found to be suitable for propagation under in vitro. Therefore, it can be used in a rapid and disease-free plantlet by in vitro culture and can be evaluated in various area, just as commercial production.
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