Micropropagation of Arugula Plant (Eruca sativa Mill.)

Abstract views: 31 / PDF downloads: 26





Callus, Growth parameters, in vitro, phytohormone, shoots, weeks


Tissue culture, an essential technique in modern plant biology, offers promising avenues for the mass production of elite plant varieties for desirable traits. This study compared the effects of 0.1 mg L-1 NAA- 0.25 mg L-1 BAP plant growth regulator combination using Cotyledon leaves (0.4 cm²) of three Eruca cultivars Estht 195, Estht 198, and Estht 201 of arugula (Eruca sativa Mill.) cultured for four and six weeks. Significant differences were noted among them for callus induction percentage, shoot induction percentage, and plant height (cm). Notably, var. Estht 201 indicated consistently improved performance, with a callus formation percentage of 61.83 % in 4 weeks and 74.05 % in 6 weeks. Similarly, Var. Estht 201 exhibited higher shoot induction (Week 4 67.45 %, Week 6:69.9 %), and plant height (Week 4:7.50 cm, Week 6:9.6 cm), throughout the experiment. These findings contributed to a deeper understanding of arugula micropropagation Dynamics. At the same time, this study has once again shown how important tissue culture micropropagation protocols are in terms of improving yield and quality as well as breeding studies.



Anonymous, 2024. PhytoTechnology Laboratories, Inc. Tissue Culture Media-Composition (http://www.phytotechlab.co m) (Accessed 10.02.2024).

Banjac, N., Krstić-Milošević, D., Mijalković, T., Petrović, M., Ćosić, T., Stanišić, M., Vinterhalter, B., 2023. In vitro shoot multiplication and regeneration of the recalcitrant rocket (Eruca sativa Mill.) variety domaća rukola. Horticulturae, 9:533.

Barazani, O., Quaye, M., Ohali, S., Barzilai, M., Kigel, J., 2012. Photothermal regulation of seed germination in natural populations of Eruca sativa Miller (Brassicaceae). Journal of Arid Environments, 85:93–96.

Freitas, E.M., Giovanelli, L.B., Delazari, F.T., Santos, M.L., Pereira, S.B., Silva, D.J.H., 2017. Arugula production as a function of irrigation depths and potassium fertilization. Revista Brasileira de Engenharia Agrícola e Ambiental, 21: 197–202.

George, E.F., 2008. Plant tissue culture procedure (Ed: E.F. George, M.A. Hall, G. Klerk). In: Plant Propagation by Tissue Culture. 3rd ed. Springer Dordrecht. pp. 1 – 28.

Hadi, M.A., Almamoori, J., Ayad, M., Al-Hassnawi Alaa, T.S., Hameedi, E.H., 2017. Oxidative response associated with treatment of male albino rats with eruca sativa mill leaves extract and correlations with complete blood picture. Journal of Pharmaceutical Sciences and Research, 9(11): 2278-2285.

Hunaish, A.A., Almasoody, M.M., 2020. Induction of callus on various explants of arugula plant (Eruca sativa Mill.) using the growth regulators (2,4-D and kinetin). Plant Archives, 20(2): 1654-1660.

Khawar, K.M., Sancak, C., Uranbey, S., Ozcan, S. 2004. Effect of thidiazuron on shoot regeneration from different explants of lentil (Lens culinaris Medik.) via organogenesis. Turkish Journal of Botany, 28(4): 421-426.

Kular, J., Kumar, S., 2011. Quantification of avoidable yield losses in oilseed brassica caused by ınsect pests. Journal of Plant Protection Research, 51: 38–43.

Küplemez, H., Yıldırım, M.U., 2020. Effects of cytokinin and auxin on plant development and vascular tissues in Lens culinaris. Commagene Journal of Biology, 4(1):16-21.

Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. physiologia Plantarum, 15(3): 473-497.

Nagar, M.M., Mekawi, E.M., 2015. Comparison of different genotypes of rocket (Eruca sativa) in terms of chemical compounds extracted from seeds and in the callus ınduced from tissue culture. Middle East Journal of Agriculture Research, 3(4): 1074-1082.

Nascimento, C.S., Nascimento, C.S., Lopes, G., Carrasco, G., Gratão, P.L., Cecílio Filho, A.B., 2022. Biofortified rocket (Eruca sativa) with selenium by using the nutrient film technique. Horticulturae, 8: 1088.

Panić, M., Stojšin, V., Budakov, D., Grahovac, M., 2021. Pseudomycosis and mycosis of rocket plant (Arugula). Biljni Lekar, 49: 38–53.

Reis, J.M., Pereira, R.J., Coelho, P.S., Leitão, J.M., 2022. Assessment of wild rocket (Diplotaxis tenuifolia (L.) DC.) germplasm accessions by NGS identified SSR and SNP markers. Plants, 11: 3482.

Jaafar, S.N.S., Jaafar, I., 2019. Eruca sativa Lınn.: Pharmacognostıcal and pharmacologıcal properties and pharmaceutical preparations. Asian Journal of Pharmaceutical and Clinical Research, 12(3): 39–45.

Soroka, J., Grenkow, L., 2013. Susceptibility of brassicaceous plants to feeding by flea beetles, Phyllotreta spp. (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 106: 2557–2567.

Srivastava, J.N., Singh, A.K., 2020. Pulses, oil seeds, narcotics, and sugar crops. In Diseases of Field Crops Diagnosis and Management. (Ed: J.N. Srivastava, A.K. Singh) Diseases of Taramira (Eruca sativa Mill.) and Their Management: Indian Perspective, Apple Academic Press: Cambridge, 2: 307-319, USA.

Su, Y.H., Liu, Y.B., Zhang, X.S., 2011. Auxin-cytokinin interaction regulates meristem development. Molecular Plant, 4(4): 616–625.

Traka, M.H., 2016. Health benefits of glucosinolates. Advances in Botanical Research, 80: 247–279.

Tripodi, P., Francese, G., Mennella, G., 2017. Rocket salad: crop description, bioactive compounds, and breeding perspectives. Advances in Horticultural Science, 31, 107–113.

Wilson, D.W., Nash, P., Buttar, H.S., Griffiths, K., Singh, R., De Meester, F., Horiuchi, R., Takahashi, T., 2017. The role of food antioxidants, benefits of functional foods, and ınfluence of feeding habits on the health of the older person: an overview. Antioxidants, 6: 81.




How to Cite

ABDUL RAUF, A., MEMON, A., & YILDIRIM, M. U. (2024). Micropropagation of Arugula Plant (Eruca sativa Mill.) . ISPEC Journal of Agricultural Sciences, 8(2), 442–448. https://doi.org/10.5281/zenodo.11278602