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Van Yuzuncu Yil University, Faculty of Agriculture, Department of Field Crops, Van
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Ankara University, Faculty of Agriculture, Department of Field Crops, Ankara
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Urmia University, Faculty of Agriculture, Department of Plant Production and Genetics, Urmia
Abstract
This study evaluated various seed treatments to identify optimal methods for enhancing the germination of Capparisspinosa (C. spinosa) seeds. Four treatments were tested: (1) sulfuric acid (98%) for 1 hour followed by 250 ppm gibberellic acid (GA3) for 1 hour; (2) soaking in water for 4 hours followed by chilling at 4°C for 24 hours; (3) soaking in water for 4 hours, chilling at -10°C for 7 days, and GA3 treatment for 1 hour; (4) soaking in hydrochloric acid (37%) for 6 hours followed by GA3 treatment for 1 hour. A completely randomized design with three replicates was used. Analysis of variance (ANOVA) revealed significant differences in germination percentage, mean germination time (MGT) at the 0.05 level, and germination rate index (GRI) at the 0.01 level. Treatments 1, 3, and 4 resulted in the highest germination percentages of 73%, 71%, and 73%, respectively, while Treatment 2 had the lowest at 51%. For MGT, Treatments 1, 2, and 3 ranked highest with values of 7.53, 7.38, and 7.21 days, respectively, whereas Treatment 4 ranked lowest at 8.4 days. The highest GRI values were observed in Treatments 1 and 3, with values of 4.89 and 5.14, respectively, and the lowest in Treatment 2 at 3.64. Results suggest that combining sulfuric acid scarification or chilling at -10 °C with GA3 significantly enhances germination. These findings provide practical insights for improving the germination of Capparis seeds, beneficial for large-scale cultivation and conservation efforts.
NAJAFI , S., ÖZCAN , S. ., & TAJBAKHSH , M. . (2025). The Effects of Prechilling, Chemical and Physical Scarification and Preheating Shock on Seed Gerimination in Capparis spinosa. ISPEC Journal of Agricultural Sciences, 9(1), 118–128. https://doi.org/10.5281/zenodo.14586268
📄Aktan, N., Bilgir, B., Elgin, E., 1981. A study on pickling and preserving caper flowers. Journal of Ege University Faculty of Agriculture, 18(1): 259-273.
📄Bewley, J.D., Black, M., 2013. Seeds: physiology of development and germination. Springer Science & Business Media.
📄Bhoyar, M.S., Mishra, G.P., Singh, R., Singh, S.B., 2010. Effects of various dormancy breaking treatments on the germination of wild caper (Capparis spinosa) seeds from the cold arid desert of trans-Himalayas. Indian Journal of Agricultural Sciences, 80(7): 621.
📄Camberato, J., Mccarty, B., 1999. Irrigation water quality: part 1. Salinity. South Carolina Turfgrass Foundation New. 6(2): 6-8.
📄Chiboub, B., Maatougui, A., Aboukhalid, K., Otouya, S., Zarqi, F., Nazih, A., Baghour, M., 2024. Effect of gibberellic acid (GA3) and temperature on seed germination of Capparis spinosa L. Journal of Applied and Natural Science, 16(1): 12-16.
📄Coşge, B., Gürbüz, B., Söyler, D., Şekeroğlu, N., 2005. Cultivation and importance of caper (Capparis spp.) (Review). Journal of Plant Research, 2: 29-35.
📄Coskun, Y., Yaman, V., 2024. Enhancing phenolic compounds in caper through the incorporation of polyamines in In Vitro cultures from various explant sources. South African Journal of Botany, 165: 43-48.
📄Güleryüz, M., Özkan, G., Ercisli, S., 2009. Caper (Capparis spp.) growing techniques and economical importance. In 1st International Symposium on Sustainable Development, Sarajevo, pp. 94-97.
📄Fivaz, A.E., 1931. Longeviety and germination of seeds of Ribes, Particularly R.rotundifolium, under Laboratory and natural conditions, USDA Technical Bulletin No, 261.40p.
📄Foschi, M.L., Juan, M., Pascual, B., Pascual-Seva, N., 2022. The imbibition, viability, and germination of caper seeds (Capparis spinosa L.) in the first year of storage. Plants, 11(2): 202.
📄IBM Corp., 2016. IBM SPSS Statistics for Windows, Version 24.0. Armonk, NY: IBM Corp.
📄Kara, Z., Ecevit, F., Karakaplan, S., 1996. Caper (Capparis spp.) as a soil conservation element and a new agricultural product. Agriculture-Environment Relations Symposium, Mersin, pp. 919-929.
📄Kontaxis, D.G., 1997. Caper: Specialty and Minor Crops Handbook. Small Farm Center,University of California, Davis.
📄Labbafi, M.R., Mehrafarin, A., Naghdibadi, H., Ghorbani, M., Tavakoli, M., 2018. Investigating the effect of various chemical and non-chemical treatments break dormancy galbanum seeds. Ferula gummosa Boiss. Ecophytochemistry journal of Medicinal Plants, 6 (2): 80-88.
📄Mazri, M.A., Koufan, M., Moussafir, S., Essatte, A., Belkoura, I., 2022. Recent advances in argan propagation: a review. Trees, 36(5): 1455-1476.
📄Ngoenngam, L., Pongtongkam, P., Arananant, J., Poeaim, S., Poeam, A., 2019. In vitro effect of gamma irradiation and plant growth regulators (PGRs) for induction and development of Stylosanthes hamata cv. Verano. International Journal of Agricultural Technology, 15(1): 63-74.
📄Nichols, M.A., Heydecker, W., 1968. Two approaches to the study of germination data. Proceedings of the International Seed Testing Association, 33: 531-40.
📄Olmez, Z., Yahyaoglu, Z., Ucler, A.O., 2004. Effects of H2SO4, KNO3, and GA3, treatments on germination of caper (Capparis ovata Desf.) seeds. Pakistan Journal of Biological Sciences, 7: 879-882.
📄Orphanos, P.I., 1983. Germination of caper (Capparis spinosa L.) seeds. Journal of Horticultural Science, 58(2): 267-270.
📄Perez, C., Pérez-García, F., Fernández, H., Revilla, M.A., 2002. The levels of GA3 and GA20 may be associated with dormancy release in Onopordum nervosum seeds. Plant Growth Regulation, 38: 141-143.
📄Pfister, R.D., 1974. Ribes L. Currant, gooseberry. In: schopmeyer CS, Coordinator. Seeds of woody plants in the United States, Washington (DC): USDA Forest Service. Agriculture Handbook No. 450. pp. 720-727.
📄Puppala, N., Fowler, J. L., 2003. Lesquerella seed pretreatment to improve germination. Industrial Crops and Products, 17(1): 61-69.
📄Soyler, D., Khawar K.M., 2006. Effects of Prechilling, Scarification, Incubation Temperature, Photoperiod, KNO3 and GA3 Treatments on Germination of Caper (Capparis ovata Desf. Var. Palaestina Zoh.) Seeds. Propagation of Ornamental Plants, 6:159-164.
📄Soyler, D., Khawar, K.M., 2007. Seed germination of caper (Capparis ovata var. Herbacea) using α naphthalene acetic acid and gibberellic acid. International Journal of Agriculture and Biology, 9(1): 35-38.
📄Sozzi, G.O., Chiesa, A.,1995. Improvement of caper (Capparis spinosa L.) seed germination by breaking seed coat-induced dormancy. Scientia Horticulturae, 62(4): 255-261.
📄Suleiman, M.K., Bhat, N.R., Abdal, M.S., Jacob, S., Thomas, R.R., Al-Dossery, S., Bellen, R., 2009. Germination studies of Capparis spinosa L. Propagation of Ornamental Plants, 9(1): 35-38.
📄Tansı, S., Çulcu, A., Nacar, Ş., 1997. Research on the germination of caper (Capparis spinosa L.) seeds. Turkey 2nd Field Crops Congress, Samsun, pp. 681-683.
📄Throneberry, G.O., Smith F.G., 1955. Relation of respiratory enzymatic activity to corn seed. Viability. Plant Physiology, 30: 337-343.
📄Zohary, M., 1960. The species of Capparis in the Mediterranean and the Near Eastern Countries. Bulletin of the Research Council of Israel, 8(2): 49-64.
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Sebahattin ÖZCAN
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